Precise transport of particles with the ability to predict the dynamic trajectory of them at single-particle and single-cell levels is important because it allows for designing crucial applications in biology and medicine. Some other magnetic manipulation methods are based on external magnetic fields, which cannot provide precise control over the particles. Current-carrying wires may also increase the chip temperature. Magnetic particle manipulation is a promising method however, in the proposed approaches based on embedded micro-coils, the wiring system is complicated. In methods based on arrays of wells, precise particle control is not offered. Moreover, due to the nature of the methods based on acoustic forces, they mostly work at the bulk level and not at the single-cell resolution. The hydraulic traps may cause undesired shear stress on trapped cells. There are multiple methods for assembling particle arrays on chips, including hydraulic traps, acoustic traps, arrays of wells, and magnetic-based techniques. However, towards this goal, an important step is to obtain a reliable technique to transport the particles of interest (e.g., cells or barcode-carrying beads) to the desired locations on the chip where various tests can be conducted. Thus, screening and diagnosis of diseases such as cancer can potentially be implemented using single-cell analysis methods based on LOC systems. Along with this concept, the lab-on-a-chip (LOC) technique has been introduced and has resulted in a revolution in medical diagnosis and treatment. In this method, even by taking much fewer samples from the body (e.g., liquid or solid biopsies) and conducting studies on a limited number of particles and cells, compared to that of the traditional methods, important rare cells can be detected and studied. The single-cell study is considered a modern approach to dealing with this problem. The introduced chip offers fundamental potential applications in the fields of single-cell biology and bioengineering. We also show a pilot mRNA-capturing experiment with barcode-carrying magnetic beads. We demonstrate the appropriate transport of both magnetic beads and magnetized living cells. The proposed magnetic transport pattern is carefully studied using both simulations and experiments for various parameters, including the magnetic field characteristics, particle size, and gap size in the design. We show that the particle transport in this system is analogous to electron transport and Ohm’s law in electrical circuits. Many particles move synced with the external rotating magnetic field, which results in highly parallel controlled particle transport. The magnetic particles repel each other to prevent undesired cluster formation. This innovative system, compared to the other rivals, offers numerous advantages. Here, we present a microfluidic platform equipped with C-shaped magnetic thin films to precisely transport magnetic particles in a tri-axial rotating magnetic field. Progress in this field requires systems capable of accurately moving the cells and particles in a controlled manner. Single-cell analysis is an emerging discipline that has shown a transformative impact in cell biology in the last decade.
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